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(3) Treatment and management Criteria The optimum dosage is 1000 g at intervals of eight weeks

Cell viability assay The cell viability was measured by the Cell Counting Kit-8 assay (CCK-8, Abbkine, BMU106-CN)

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Testicular histology was assessed using Hematoxylin-Eosin staining to observe seminiferous tubule morphology, and a Johnsons score was applied to quantify spermatogenic function based on a 10-point scale