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(Li et al., 2017) demonstrated that puerarin significantly enhanced ABCA1 mRNA and protein expression in human THP-1 macrophage-derived foam cells through activation of AMPK, the peroxisome proliferator-activated receptor gamma (PPAR-) and LXR- pathways

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After 24 h, the medium was replaced with fresh medium containing FBS, and puromycin (30 g/mL