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At each time point, 20 L of iHLO culture medium was transferred to a 384-well white flat-bottom plate, 20 L of Luciferin Detection Reagent was added, and the mixture was incubated for 30 min before measuring luminescence

FOXO4-DRI prompted relocation of p53-pS15 and FOXO4 in the serum limitation-induced senescent fibroblast model Double immunofluorescence staining was performed to investigate the distribution of p53-pS15 and FOXO4 in serum limitation-induced senescent fibroblasts model with or without treatment with FOXO4-DRI (Fig