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After that, the PVDF membranes were washed with TBST for four times, and the blot was developed using ECL reagent according to the manufacturers protocol

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An in vitro model of cerebral Ischemia-Reperfusion was established using oxygen-glucose deprivation and reoxygenation (OGD/R)

Thus, we calculated HPA axis rhythm measures based on cortisol levels at 3 time points, AUC 08:0016:00 , AUC 16:0023:00 and AUC 08:0023:00 , as well as DCS

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