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The antibodies for GGT (1:1000, ab109427, rabbit mAb), GPX4 (1:1000, ab125066, rabbit mAb) and -actin (1:1000, ab8226, mouse mAb), goat anti-rabbit IgG H&L (HRP) (1:5000, ab6721), and goat anti-mouse IgG H&L (HRP) (1:5000, ab205719) were used and obtained from Abcam

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Afterward, the lysosomes of cells were stained with LysoTracker Green and the mitochondria of cells were stained with MitoTracker Green at 37 C for 30 min