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Co-immunoprecipitation with protein A or protein G Materials Immunoprecipitation Starter Pack (contains nProtein A Sepharose 4 Fast Flow and Protein G Sepharose 4 Fast Flow) Lysis buffers Other buffers PBS: 1 mM KH 2 PO 4 , 10 mM Na 2 HPO 4 , 137 mM NaCl, 2.7 mM KCl, pH 7.4 Wash buffer: 50 mM Tris, pH 8 Sample buffer: 1% SDS, 100 mM DTT, 50 mM Tris, pH 7.5 (reducing) Media preparation nProtein A Sepharose 4 Fast Flow and Protein G Sepharose 4 Fast Flow are supplied preswollen in 20% ethanol