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The combination index (CI) at the IC 50 level was determined using Compusyn software based on the ChouTalalay method to evaluate drug interaction effects

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Bioinformatics analysis Correlation analysis Re-processed RNA-Sequencing counts and metadata were generated by the authors of the ARCHS4 repository as described in their publication 108

The obtained recA gene sequence of the selected isolates was compared with those in the GenBank database using the nucleotide BLAST tool ( for the identification of the test isolates using the parameters of percentage identity more than 95% and E-value equal to zero then the phylogenetic tree was assembled for each of the tested isolates sequences using the neighbor-joining algorithm in the nucleotide BLAST tool

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Additionally, variations in dietary composition, particularly in terms of crude protein (CP) content and the balance of other essential AA like lysine (Lys), can yield differing productivity responses