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Flow cytometry For viability staining, CD4 + T cells were washed with PBS then stained with Zombie viability dyes (BioLegend) at room temperature for 5 min, and then washed with FACS buffer

Per FDA guidance, GLP-1-based medications are generally approved for adults with a BMI over 30, or over 27 with a qualifying health condition such as type 2 diabetes, high blood pressure, or high cholesterol

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Potential side effects of B12 injections are generally mild but should be monitored

If you are asymptomatic, we will usually suggest oral supplementation before any injection