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The stranded mRNA-seq library was generated from 1000 ng of total RNA from each sample using Truseq stranded mRNA library kit (Illumina) according to the product instructions

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4.1 Complexity of GB biology Intra-tumoral heterogeneity, which has also been previously described in GB using single-cell RNA-seq profiling ( Redundant signaling pathways in gliomagenesis limit the effectiveness of targeted therapies that focus on a single gene or pathway, even when the tumors complete molecular profile is known