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doi: 10.1016/j.bbrc.2013.08.090 351 PagliaruloCDe VitoVPicarielloGColicchioRPastoreGSalvatorePet al

While I recommend starting low (1 to 10 billion CFUs per day) and increasing slowly, doses of at least 50 billion CFUs per day are needed for therapeutic effect

The resulting mixture was incubated for 1 h at -20C to precipitate residual salts from the chemically defined medium

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