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In addition, iron acts as the active center of lipoxygenase, which oxidizes polyunsaturated fatty acids on cell membranes through an iron-ion-dependent chain reaction, thereby promoting the proliferation of phospholipid peroxides and thus causing ferroptosis

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Superoxide anions level was expressed as nanomoles of 2-hydroxyethidium (2-OHE), and the calibration curve was prepared using the extinction coefficient of 2-OHE ( = 9.4 mM 1 cm 1 )

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The cells were stimulated by either lipopolysaccharide (LPS) (SigmaAldrich, USA) or staphylococcal enterotoxin B (SEB) (Toxin Technology, Sarasota, FL, USA) with or without crocin (target mol, USA) when they reached 70-80% confluency

In particular, mitochondrial redox reactions are a major source of ROS in most cells, including tumor cells [28]