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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

At its IC50 concentration, vincristine induced significant downregulation of Ribonucleotide Reductase M2 (RRM2), involved in DNA damage repair and cell cycle regulation ( Supplementary Table S1 )

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