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Cellular ROS detection assay Following spheroids treatment with different Vit-C doses for 72 h, the cellular ROS was determined by performing the ROS-GLO H 2 O 2 ASSAY (PROYY-G8820, Promega, USA) according to the protocol from the manufacturer

& Sziklai-Lszl, I.) (1998)

it away from heat, moisture, and direct sunlight to preserve its stability

Data analyses All data are presented as mean SEM and were analyzed by one-way and two-way ANOVA followed by Bonferroni corrections for multiple comparisons between means using the error mean square terms from each ANOVA analysis as described previously 36

G protein-coupled receptor 43 moderates gut inflammation through cytokine regulation from mononuclear cells

The harvested bacterial cells were suspended in TBS buffer