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Cytokine Control of Inflammation and Repair in the Pathology of Multiple Sclerosis

HCT116 Flp-in cells (seeded in 10 cm plates, 30% confluency) were co-transfected with 9 g of donor plasmid and 3 g of sgRNA-Cas9 plasmid, and 24 l Turbofect (Thermo Scientific, R0533) in 2 ml Opti-MEM (Thermo Scientific, 11058-021)

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EGCG has been shown to safeguard mitochondrial function by preventing Ca2+ influx through voltage-gated calcium channels and mitochondrial Ca2+ uptake via the mitochondrial Ca2+ uniporter (159, 193)