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J Med Genet 41(4):e38 Del-Bel E, De-Miguel FF (2018) Extrasynaptic neurotransmission mediated by exocytosis and diffusive release of transmitter substances

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coli GI826 competent cells, which were then cultured in 50 ml of IMC medium (1 M9 salts, 0.2% casamino acid, 0.5% glucose, 1 mM MgCl 2 ) containing 100 g/ml ampicillin with shaking (225250 rpm) to saturation (OD 600 = 3) for 15 hours at 25C

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