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The global burden of BD is substantial

The second coating preferably surrounds (or completely covers) and contains the first coating

2024;10:eadm9859

To construct a random mutagenesis library based on the HpaB mutant 23F9, Error-prone PCR was performed using plasmid pFA- hpaB 23F9 C as template with primers hpaB -ErrorProne-for and hpaB -ErrorProne-rev

We hypothesize that the failure of intrinsic-solubility approaches arises from inaccuracies in the predicted valuesalthough Starling is a state-of-the-art p K a -prediction model, it still routinely predicts values that are wrong by 0.51.0 p K a unit, which can correspond to a 10x difference in the predicted value

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