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After 3 h for cell attachment, medium was exchanged to Sera Plus-free medium and cells were allowed to adjust for 16 h before treatment

If IGF-1 remains unchanged, it suggests poor timing, inadequate dosing, or receptor resistance

This study investigated the role of irisin in an MPTP-induced mouse model of PD

(PubMed) Perrig WJ, Perrig P, Stahelin HB