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Depress the plunger slowly at a rate of 1ml/10 seconds

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Remove supernatant and resuspended cells (bead-bound) with dig wash buffer (20 mM HEPES pH 7.5

To further investigate the relationship between DHA and risk for dementia, analyses of dietary DHA and fish intake were performed in the subsample of 488 participants who completed the semiquantitative food frequency questionnaire at the beginning of the follow-up period, as shown in Table 4

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