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To rule out the possibility that EMS-induced mutations in genes other than Ppa-rme-2 have resulted in the observed Tid phenotype, we generated an additional knock-out mutation by CRISPR engineering

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Historical Background and Scientific Context Research into synthetic peptides expanded rapidly during the late twentieth century as advances in peptide synthesis enabled scientists to replicate biologically active fragments from larger proteins

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Importantly, 5MQ was found to effectively inhibit HeLa cell proliferation without apparently affecting normal HEK-293 cell proliferation suggesting selectivity for cancer cell biology contexts