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Small modifications to this system allowed the generation of branching organoids 23 , akin to the morphogenesis of the developing tissue 24,25 , and organoids could be transplanted into animals to reconstruct the bile duct in vivo 26

pneumoniae in host cells We have demonstrated that the pneumococcus can survive inside pneumocytes, macrophages and neutrophils for several hours [8]

In vivo chemogenetic manipulations For chemogenetic experiments in the DMV, rAAV-Ef1-DIO-hM3D(Gq)- mCherry-WPRE-pA (AAV-DIO-hM3Dq-mCherry, AAV2/9, 5.00 10 12 vg/mL) or rAAV-Ef1-DIO-hM4D(Gi)-mCherry-WPREs (AAV-DIO-hM4Di-mCherry, AAV2/9, 2.25 10 12 vg/mL) was injected into the DMV of C57 mice

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While both influence neural pathways, they are designed with different primary objectives