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The first incorporates GSH into the mitochondria by inorganic phosphate exchange and the second by exchange of 2-oxoglutarate (27, 28, 64) (Figures 2, 4)

The filtrate is evaporated and the glutathione is purified by recrystallization from 50% ethanol

This led on to methods that require alkylation of the reactive sulfur atom as the trapping reaction 28

In this approach, the gene coding for glutamate decarboxylase (GAD), which converts glutamate (the major excitatory neurotransmitter) into GABA (the major inhibitory neurotransmitter) is delivered to the subthalamic nucleus (STN)

Diabetic retinopathy Chronic hyperglycemia can damage blood vessels in the retina and cause a potentially sight-threatening condition called diabetic retinopathy (83)

Different cell types have significantly different cystine uptake capacities, and cell types with low uptake capacity may exhibit a relatively high background