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McCoy and colleagues reported that 5-day treatment of dissociated rat hippocampal neurons with Dihexa increased dendritic spine density approximately three-fold compared to vehicle control (~41 spines per 50 m of dendrite versus ~15 spines), with the increase reflecting both new spine formation and increased spine-head width consistent with synaptic maturation [2]

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The following compounds were purchased from the indicated suppliers for in vitro studies: FK-866 (#HY-50876, MedChemExpress, NJ, USA), GNE-617 (#HY-15766, MedChemExpress), GNE-618 (#HY-12628, MedChemExpress), disulfiram (#PHR1690-1G, Millipore Sigma), -nicotinamide mononucleotide (NMN) (#N3501-25MG, Millipore Sigma), and nicotinic acid (NA) (#N4126-100G, Millipore Sigma)

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As NAFLD condition increased oxidative stress liver homogenate Superoxide Dismutase (SOD), reduced Glutathione (GSH) level was significantly decreased and Lipid Peroxidation (LPO) level significantly increased in NAFLD control compare with normal control

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