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Co-immunoprecipitation (Co-IP) The cell supernatant lysates were incubated with 2 g of antibody and 20 l of protein A or protein G sepharose beads overnight at 4 C, and further experiments were performed with an immunoprecipitation kit (Beyotime) according to the manufacturers protocol

[DOI] [PMC free article] [PubMed] [Google Scholar] 212.Hira S., Saleem U., Anwar F., Sohail M.F., Raza Z., Ahmad B