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After removal of the overlying meninges and blood vessels, spinal cords were cut into small pieces, dissociated with 0.05% collagenase I (Thermo Fisher Scientific, Foster City, CA, USA) for 15 min at 37 C, and then processed to produce epSPC neurospheres as previously described 6,18

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Hypertension plays a cardinal role in the progression of cerebromicrovascular injury and vascular cognitive impairment [42]

A European cohort from Erasmus MC (Netherlands) showed that overweight, diabetes, hypertension, and dyslipidemia independently increased liver-related events, including HCC, transplantation, and mortality, with multiple comorbidities amplifying risk several-fold across subgroups, including virally suppressed and non-cirrhotic patients (94)