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Material and methods Animals The Eighteen Olkuska sheep were selected for the study, 9 of which were ewes at the age of 2 years (multiparas) and 9 were 14 months of age (primiparas) whose selection was based on type of birth (maximum 5 day difference between partiturations and only twin births) and health status

The same mixture containing the target protein inactivated by boiling served as the control ( m ) and maximum velocity (V max ) were determined by fitting the initial velocity data to the MichaelisMenten equation using nonlinear regression analysis in Origin software (OriginLab Corporation, Northampton, MA, USA)

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Over two months, participants received daily doses of 400 milligrams -tocopherol (vitamin E), 40 milligrams -carotene and 1,000 milligrams vitamin C

Skipping the temperature equilibration step: Cold-to-warm condensation can introduce moisture before reconstitution begins

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