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Availability: InStock
Rating: 4.3537 (14 reviews)

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The 1H NMR facility was funded by Science Foundation Ireland 2012 Strategic Opportunity Fund (Infrastructure award 12/RI/2346/SOF)

Sci Rep (2018) 8(1):11873

Merrifield and Carnevali, 2014)

Soak: Place the mung beans and chickpeas in a large bowl and cover with room-temperature water, about 2 inches above the legumes

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To test the ability of LnaB and MavL to convert PR-Ub into active ubiquitin, 1.5 g His 6 -LnaB, 1.5 g Actin, 1.5 g GST-MavL and 4 g PR-Ub were preincubated in a 25 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 and 1 mM ATP for 1 h at 37 C