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The tubes were then rotated overnight at 4 C, followed by incubation with protein A/G beads under the same condition overnight

Biochem Biophys Res Commun 71(4):952958 Maiorino M, Bosello V, Cozza G, Roveri A, Toppo S, Ursini F

As noted in section V.K.6.b.(1), DES is a good indicator of socioeconomic disadvantage, as dual eligibility is associated with a patient's inability to access care

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Sometimes longer, depending on dosage and health goals