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The dsDNA handle was prepared by PCR (see Supplementary Table 3 for oligonucleotides) including 200 M final concentration of each dNTP (dGTP, dCTP, dATP), 140 M dTTP and 66 M Bio-16-dUTP (Roche) using the plasmid pSP73-JY0 as template 80 followed by digestion with the restriction enzyme BsaI

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The 1:1 mass ratio (5mg each) in this vial provides approximately 3.5 nmol of BPC-157 for every 1 nmol of TB-500, reflecting the molar ratios used in the majority of combination research protocols