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Importantly, this effect was reversed when cells were cultured with supernatant from chelator-treated macrophages [64]

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Oxidative stress in aging Aging is defined as an intrinsic, multifactorial, and progressive process characterized by tissue degeneration and progressive loss of organ function, ultimately leading to increased mortality (Liguori et al., 2018

Defining symptom targets (such as energy levels, sleep quality, or recovery time), safety thresholds for side effects, and measurable progress markers helps clinicians tailor protocols effectively

While the study reported positive effects on cerebrospinal fluid biomarkers of neuroinflammation and neurodegeneration, it did not meet its primary clinical efficacy endpoint, highlighting the difficulty of translating biological signals into measurable clinical benefits in a heterogeneous patient population (65)

Nguyen V T, Huynh A T and Dao T S 2022 Acute and chronic toxicity of mixtures of bisphenol A and trace metals (Cd and Pb) to micro-crustacean, Daphnia magna