A construct of pcDNA3.1(+) GSTP1 containing a V5 tag at the COOH-terminus [pcDNA3.1(+)-GSTP1_V5] was generated by PCR amplification of pcDNA3.1(+)-GSTP1 using the forward primer 5TACCGAGCTC GGATCC GCCATGCCGCCGTATACCGTG 3with a BamHI restriction site indicated in italics and the GSTP1 sequence in bold
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The suppression of prolactin is required for the treatment of advanced prostate cancer
In conclusion, the crystal structures of Lgt reveal the molecular basis of several functional characteristics of this essential membrane enzyme
