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shaking creates foam and mechanical stress that breaks peptide bonds

A widely used research tool for studying IGF-1 receptor (IGF-1R) signal transduction including PI3K/Akt pathway activation, MAPK/ERK phosphorylation cascades, and downstream transcription factor engagement in IGF-1R-expressing cell lines

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Biochem Biophys Res Commun (2010) 403:249.10.1016/j.bbrc.2010.10.100 65 CassoniPGheCMarroccoTTarabraEAlliaECatapanoFet alExpression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines

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They cannot be weighed on the same scale