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During elution in this method, ionic strength increases, and pH changes occur

Despite that, a notable observation was made regarding the -cyano acrylamide CA007

Data extraction was carried out using a data extraction form which was designed using the Joanna Briggs Institute (JBI) guidance on data extraction (Tufanaru et al ., Reference Tufanaru, Munn, Aromataris, Campbell, Hopp, Aromataris and Munnz2020) and the Cochrane Handbook for Systematic Reviews of Interventions (Li et al ., Reference Li, Higgins, Deeks, Higgins, Thomas, Chandler, Cumpston, Li, Page and Welch2019)

Nevertheless, there are still some disadvantages of these techniques, for example, non-specific cross-hybridization usually cause a high background level which limits the detection range, the transcripts can be detected only with high copy number, and the total coverage of the transcripts are almost unknown (Liu et al., 2015)

10.3389/fmicb.2018.02700 28 BurdetteD

Both work, but they absorb differently