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Next, the samples were concentrated in SpeedVac (Savant) under vacuum for 40 min, and the concentrated samples were re-constituted to the original volume by 10 l of Millipore water

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PBMCs were obtained via density-gradient separation and were either used fresh or cryopreserved in FBS supplemented with 10% DMSO

& Dianov, G

13 showed that GLS was overexpressed in the livers of NASH patients and in mice in diet-induced NASH models, providing evidence that glutamine metabolism is a potential therapeutic target in NASH progression

In addition, cataractous patients in the study had a greater intake of animal foods and fried snacks