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The next day, after washing with TBST, it was incubated for 1 hour with the HRP-conjugated secondary antibody at room temperature

Thaler, J

Arabidopsis protoplasts were obtained from leaves of 4-week-old plantlets expressing the NADP(H) biosensor directed to chloroplasts or cytosol grown under Arabidopsis chamber conditions in Magenta boxes containing SCA medium (0.32% Gamborgs basal salt powder with vitamins, 4 mM MgSO 4 .7H 2 O, 43.8 mM sucrose and 0.15% (w/v) phytoagar, pH 5.8) supplemented with 30 g/l kanamycin

In addition, because SCHs and MDHs use the same MS-DRGs as other hospitals when they are paid based in whole or in part on the hospital-specific rate, the hospital-specific rate is adjusted by a budget neutrality factor to ensure that changes to the MS-DRG classifications and the recalibration of the MS-DRG relative weights are made in a manner so that aggregate IPPS payments are unaffected