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4 Immunometabolic dysregulation in autoimmune diseases Autoimmune diseases arise from a loss of self-tolerance and aberrant immune activation, with T cells playing a central role in their pathogenesis

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Conversely, the amino acid-based elution buffer (50 mM Leucine-HCl, pH 3.5) resulted in an increase in pH values and decent conductivity of the elution fraction, as well as a doubling in the column volume (CV) which indicating a progressive elution (Figure 2)