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For the analysis of PTP oxidation, frozen liver samples were homogenised under anaerobic conditions in the presence of N -ethylmaleimide to alkylate all reduced PTPs and then subsequently reduced and hyperoxidised to the sulfonic state as described previously [13]

Moreover, it is noteworthy that nanotechnology offers immense potential in preventing and alleviating PIPN via two main strategies: 1) enhancing the targeting accumulation and/or activity of Pt drugs at tumor tissues while decreasing the off-target effects and 2) specific delivery of antioxidant agents toward the peripheral nervous system

Berthold EC, Kamble SH, Raju KS, King TI, Popa R, Sharma A, Len F, Avery BA, McMahon LR, McCurdy CR., Preclinical pharmacokinetic study of speciociliatine, a kratom alkaloid, in rats using an UPLC-MS/MS method, J Pharm Biomed Anal

Critically, in vitro binding or degradation does not guarantee efficacy in vivo due to the complex gastrointestinal environment, including variable pH, proteolytic enzymes, bile salts, and competition from resident microbiota (30, 94)

Cell 184 (23), 57915806.e19