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Structural and mechanistic insights into LEOPARD syndrome-associated SHP2 mutations

We utilize three imaging families based on imaging modality for purposes of this methodology: (1) ultrasound

For general recovery, standard abdominal subcutaneous injection works well

The sample was then centrifuged (Eppendorf 5810R, Hamburg, Germany) for 20 min (3220 g , RT) and the supernatant was collected and paper filtered (Whatman 597 1/2 ) for immunoaffinity purification

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The benzyl alcohol acts as a preservative, allowing multiple sterile withdrawals from the same vial