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Factors such as high pressure and speed, during sorting, dye-induced defects, electrical deviation, changes in pH and osmolarity, etc might lead to changes to the sperm plasma membrane, trigger pre capacitation-like changes, cause protamine decondensation and alter sperm DNA quality (68)

MK-677 (half-life 24 hours) requires a longer taper 3-4 weeks minimum because its prolonged receptor occupancy causes deeper suppression that takes 28-35 days to fully resolve

MAST4-sh2 slightly affected the ciliary assembly

Biol Trace Elem Res 76(2):161173

Biol 12 , 817 (2017)

This damage was associated with significant lipid peroxidation, which could be mitigated by the antioxidants trolox, butylated hydroxytoluene, N-acetylcysteine, and membrane-permeable iron chelators such as 2,2-dipyridyl, 1,10-phenanthroline, LK614 LK616, and deferoxamine