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In a differential alkylation approach, peptides containing Cys (either chemically alkylated or initially oxidized) are used to estimate Cys oxidation levels, while peptides not containing Cys are used for measurement of protein expression

We applied counterfactual mediation analysis to estimate the direct and indirect effects of genetic variants on cirrhosis through mediators including metabolic dysfunction, alcohol use, viral hepatitis, and other causes of liver disease in 11,738 cirrhosis cases and 444,882 controls using individual-level longitudinal electronic health record (EHR) data from MVP

Vox Sang 112:326335

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These results suggest that Fasn inhibition reduces ROS levels via the Nrf2/HO-1 pathway, thereby preventing NLRP3 inflammasome activation and mitigating the inflammatory response and cell pyroptosis induced by Ang II

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