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10.1038/9045 117 WangH.RaoB.LouJ.LiJ.LiuZ.LiA.et al (2020)

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Blood was flushed out with 150 mL of saline with 100 U/L heparin at 30 mL/min, followed by perfusion with 3% formaldehyde and 150 mL of 1% glutaraldehyde solution

Because of the crucial role of the intestinal microbiota in host homeostasis, it is important to study the age-related differences in microbiota and how it influences intestinal function