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Briefly, the intensity, as determined by the area under the peak, of all peaks relating to SOD1 monomers ( I M ) and dimers ( I D ) were summed to give total signal intensity ( I T ): The proportion of monomer signal ( P M ) was determined by dividing the intensity of the monomeric species ( I M ) by the total signal intensity ( I T ): The concentration of monomer at equilibrium ([ M ] eq ) was determined by multiplying the proportion of monomer signal ( P M ) by the total protein concentration in micromolar ([ P 0 ]): The concentration of dimer at equilibrium ([ D ] eq ) is defined as K d values were derived from the slope of a linear regression fit of the plot of [ D ] eq against [ M ] 2 eq (fitting performed with Prism 5.0, GraphPad Software, Inc.) (Supplementary Fig

This process is known as genomic or nuclear response and entails up- and down-regulation of diverse genes associated with cell proliferation, survival, and migration (Musgrove and Sutherland, 2009

Considering that current cancer diagnoses primarily rely on features observed in cancer cells at primary sites, gaining a deeper understanding of the lipidomic and metabolic adaptations that occur during LN metastasis could offer novel solutions to prevent the spread of cancer cells

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