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To remove H 2 O 2 excess, GPx, TrxR, and CAT (i.e., enzymes involved in EAS) are dependent on reduced nicotinamide adenine dinucleotide phosphate (NAPDH) for the renewal of its substrate, thereby enabling the clearance of ROS

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Brown, M.J

The negative slope value of sleep-deprived mice treated with GHK (SD + GHK) was similar to the negative slope value of non-sleep-deprived mice treated with saline (Control)

Strategies to minimize reactions include rotating injection sites consistently to avoid repeated trauma to the same area, ensuring the solution is at room temperature before injection (cold injections can increase irritation), injecting slowly rather than quickly, and using a smaller gauge needle for less tissue trauma