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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat

While sixteen metabolites were conserved across species, four O -methylated and hydroxylated catechol metabolites were detected exclusively in rodents, and molecular networking revealed higher overall metabolite formation in rat hepatocytes

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These studies indicate a link between the diet and the gut microbiota