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Immunofluorescence staining and analysis For visualization of RNH1(D210N)-GFP foci and H2AX foci, cells grown on autoclaved coverslips were washed with ice-cold PBS and pre-extracted for 5 min with CSK [25 mM HEPESNaOH (pH 7.7), 50 mM NaCl, 1 mM EDTA, 3 mM MgCl 2 , 0.3 M sucrose, 0.5% (v/v) Triton X-100] buffer on ice

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B12 plays many important roles in the human body, including: Nerve function Brain health Red blood cell production DNA synthesis Energy production Some people simply do not get enough Vitamin B12 in their diet to meet the bodys demands, while others have difficulty absorbing it

Dietary B12 deficiency comes from inadequate intake, common in strict plant-based diets

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