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The resulting PCR product was digested with NdeI and EcoRI and cloned along with the EcoRI/BspEI fragment of pQE60-yRad52-Tat11-His 6 59 in the plasmid pTXB1 (New England Biolabs) to create a bacterial expression vector for RNH1(D210N)-GFP-Tat11-His6 fusion protein

Thus, the GSH/GSSG ratio reflects the oxidative state and can interact with redox couples to maintain appropriate redox balance in the cell

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