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doi: 10.1074/jbc.M109.081125 133 SllvanderSNikitidouEBrolinRSderbergLSehlinDLannfeltLet al

10.1016/j.jchf.2015.03.007 52

Liew Z, Bach CC, Asarnow RF, Ritz B, Olsen J

These enriched extracts are useful when a protein of interest is necessary for a downstream application or when it simplifies the reaction setup

During protein folding stress, the sirtuin SIRT7 also plays an important role by contributing to repressing nuclear respiratory factor 1 (NRF1) activity, a major transcriptional regulator of mitochondrial genes

156 However, changes to expedite extract production such as low-speed clarification steps (S12) and the absence of the dialysis step, have changed the concentration of endogenous components in the final extract