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The other two detected cysteines, C87 and C252, were not methylated, while cysteines forming the active site, C157 and C207, were not detected in our assay, even with various proteinase digestion methods

J Pineal Res 65(4):e12520 Daz Lpez B, Rodrguez ED, Urquijo C, Alvarez CF (2005) Melatonin influences on the neuroendocrine-reproductive axis

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There were some limitations in this study including the measurement of serum glutamine and the absence of proper cooperation on the part of some participants during the study

Heat inactivated serum was included as negative control

Furthermore, none of the compounds showed hERG inhibition or hepatotoxicity, except for compound 4, which was predicted to be potentially hepatotoxic