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The cells were fixed with 4% PFA and 0.2% glutaraldehyde in a buffer containing 30 mM HEPES (pH 7.4), 100 mM NaCl, and 2 mM CaCl 2 for 10 min at room temperature

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Lipid peroxides released early during ferroptosis act as damage-associated molecular patterns (DAMPs), promoting dendritic cell (DC) maturation and antigen cross-presentation, which in turn primes CD8 + T cells and enhances anti-tumor immunity ( + T cells and DCs, thereby positioning CDH4 as a promising target for combination therapies leveraging ferroptosis and immunotherapy

[Google Scholar] [152].Nakajima M, Yamaguchi I, Purification of plant hormones by immunoaffinity chromatography, Kagaku Seibutsu 29 (1991) 270275

Next, fluoroscopy (x-ray) or ultrasound is used to find the target