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Thus, displaying that -mRNA translational increase is dependent on PKR activity [ in vivo studies, 8-week-old BALB/c mice (weighing 1823 g) was treated with 3.0 g of -modified mRNA and unmodified mRNA it was observed that, unmodified mRNA induced high levels of IFN- in serum while -modified mRNA did not induce higher level of IFN-, 12-fold higher protein expression was observed [ Following the Kariko paradigm, Andries et al