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J Int Soc Sports Nutr 2015 , 12 (1)

After 0 h, 24 h, 48 h, 72 h, and 96 h of inoculation, the CCK-8 reaction solution was added according to the instructions of the CCK-8 kit (Beyotime, Shanghai, China), and the absorbance value at the wavelength of 450 nm was detected using an TECAN reader after incubation at 37 C for 1 h

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Y.et al (1998)

and (c) they show expected responses to treatments that are effective in the human disease (predictive validity)

If the amino acid has a side chain that also has a pKa (pKa 3 in the table above), the isoelectric point is determined by taking the average of the two closest pKa values